Visualization of central noradrenergic neurons in thick sections by the unlabeled antibody method: a transmitter-specific Golgi image.
- 1 May 1978
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 75 (5) , 2502-2506
- https://doi.org/10.1073/pnas.75.5.2502
Abstract
The unlabeled peroxidase-antiperoxidase method was used with a guinea pig antiserum vs rat dopamine-.beta.-hydroxylase (DBH) to obtain a 3-dimensional image of noradrenergic cell bodies and their processes in thick Vibratome sections of rat brain. This method stains DBH-positive neurons exclusively with a result similar to that of the Golgi method, which makes it possible to analyze the geometric plan of these neurons and their projections in the CNS. In 100 .mu. sections, DBH-positive axons can be followed over long distances, and the results indicate that their distribution in cerebral and cerebellar cortex is not diffuse but has a strict geometric order.This publication has 18 references indexed in Scilit:
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