THE EFFECT OF ACETYLCHOLINE CONCENTRATION ON MEMBRANE POTENTIAL, REDOX STATE AND SECRETION OF THE PANCREATIC ACINAR CELL

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Abstract
Continuous stimulation of the isolated perfused rat pancreas with acetylcholine (ACh) at a low concentration (5 .times. 10-8 M) induced flat increases in secretory responses, which were decreased to values of .apprx. 30% control obtained in the standard medium when Ca was omitted from the perfusing and bathing solution. The secretory responses were associated with little or no change in the membrane potential and the redox state of the acinar cells. Continuous stimulation with ACh at a moderate concentration (3 .times. 10-7 M) induced secretory responses which consisted of the following 3 phases: initial rise, 2nd plateau and the final decline. When Ca was removed from the perfusing and bathing solution, the initial phase was decreased to .apprx. 1/2 of the control obtained in the standard medium, and the 2nd and the final phases were strongly inhibited. Continuous stimulation with 3 .times. 10-7 M ACh caused only a transient small depolarization of the acinar cell and a gradual reduction in the redox state. Continuous stimulation with ACh at a higher concentration (10-6 M) induced a rather small flat increase in amylase output, which was further enhanced when Ca was removed from the perfusing and bathing solution. Continuous stimulation with 10-6 M ACh caused a transient oxidation followed by a gradual reduction in the redox state.

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