Cellular composition of milky spots in the human greater omentum: An immunochemical and ultrastructural study

Publisher Website
Google Scholar
Abstract
Background: Milky spots in the greater omentum of some animals are well organized perivascular infiltrated of leucocytes, and are considered to have characteristics of secondary lymphoid tissue. To determine whether milky spots in the human greater omentum can also be regarded as secondary lymphoid tissue, we studied milky spots in an unstimulated state. Methods: Patients were selected on the basis of absence of disease in the peritoneal cavity that might influence the state of the milky spots. Using monoclonel antibodies aganist macrophages, B-lymphocytes and T-lymphocytes, and immunoperoxidase labeling, the number of these cells and their location in milky spots were studied by light microscopy. However, the stromal components of the greater omentum, especially those within the milky spots, were studied by electron microscopy. Results: Milky spots in the human greater omentum are relatively uniform vascularized accumulations of mononuclear cells comprising macrophages (67.9% ± 9.4, mean ± standard deviation), B-cells (10.1% ± 3.4), T-cells (10.2% ± 3.7), and mast cells. However, no special B-cells and T-cell areas could be distinguished. On the ultrastructural level it was demonstrated that macrophages are present in different stages of maturation and can enter or leave the milky spots. Furthermore, no cells characteristic of secondary lymphoid organs, such as interdigitating cells or follicular dendritic cells, were seen. Conclusions: These data indicate that unstimulated milky spots in the human greater omentum are to a great extent just a preformed specific accumulation of primarily macrophages within the stroma of the greater omentum, and therefore, cannot be regarded as true secondary lymphoid tissue. Milky spots could serve as a gateway for, as well as a provider of pertioneal macrophages when the intra-abdominal status so requires. Finally, the data from this study are compard with the data of other studies of human milky spots and those in animals.