Shufflon: multi-inversion of four contiguous DNA segments of plasmid R64 creates seven different open reading frames
Open Access
- 11 February 1987
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 15 (3) , 1165-1172
- https://doi.org/10.1093/nar/15.3.1165
Abstract
The IncIα plasmid R64 was found to bear a highly mobile DNA segment which was designated as a clustered inversion region (J. Bacteriol. 165, 94–100, 1986). The clustered inversion region consists of four DNA segments designated respectively as A, B, C and D which differ in molecular size and restriction sites. The four DNA segments invert independently or in groups resulting in a complex DNA rearrangement. We now show the nucleotide sequence of the clustered inversion region of R6A. The present results suggest that the clustered inversion region is a biological switch to select one of seven open reading frames whose primary structures at the region proximal to N-termini are constant while those at the C-terminal region are variable. A name, “Shufflon” was proposed to call this kind of the clustered inversion region.Keywords
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