Establishment of a Chicken × Chicken Hybridoma Secreting Specific Antibody
- 1 January 1989
- journal article
- research article
- Published by S. Karger AG in International Archives of Allergy and Immunology
- Vol. 89 (4) , 416-419
- https://doi.org/10.1159/000234985
Abstract
Chicken B lymphoblastoid cell mutants lacking thymidine kinase (TK) were selected from the parental cell line treated with ethyl methanesulfonate. To obtain chicken hybridomas secreting immunoglobulin against the defined antigen, a TK–– mutant was fused with inactivated Newcastle disease virus (NDV)-immunized chicken spleen cells using polyethyleneglycol. Finally, one hybridoma secreting IgM specific for NDV was established.This publication has 4 references indexed in Scilit:
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