Studies of the Macrophage Inhibition Test

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Abstract
Summary: The course of development of delayed skin reactivity in vivo and inhibition of peritoneal cell migration in vitro were compared in guinea pigs vaccinated with diphtheria toxoid (Dtd) and human γ globulin (HGG) in complete Freund's adjuvant. A modification of the capillary tube method of migration-inhibition provided a simple and reproducible system that was susceptible of statistically analysis and that allowed the detection of low levels of reactivity. Delayed skin reactivity appeared earlier and developed more rapidly than migration-inhibition in the first 2 weeks after immunization. Studies with graded doses of Dtd later after sensitization indicated that, once sensitivity was well established, the in vivo and in vitro reactivities followed the same course. Arthus reactivity in skin test sites did not coincide with maximum delayed skin reactivity or migration-inhibition.

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