Adaptation of Coenzyme Stimulation Assays for the Nutritional Assessment of Vitamins B1, B2 and B6 Using the Cobas Bio Centrifugal Analyser

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Abstract
Adaptation of coenzyme stimulation assays for the nutritional assessment of thiamine, riboflavin and pyridoxine on the Cobas Bio centrifugal analyser are described. Whole blood was collected into acid-citrate dextrose, which preserves the erythrocytes, prior to assay for several days. Washed erythrocytes stored at −70°C and subsequently thawed, showed altered enzyme activities. The methods offer improved precision over existing procedures and take advantage of the high throughput capabilities of the instrumentation.