Production in Rabbits of Immune Tolerance to a Mixture of Antigens.

Publisher Website
Google Scholar
Abstract
In 2 litters, totaling 9 rabbits, immune tolerance to the antigens in normal human blood was induced by a series of injections. On the day of birth, 1 ml of normal human blood, fortified with added buffy coat, was injected intraperitoneally into each rabbit. Two additional similar injections were given during the neonatal period. At the age of 3 months, both treated and control rabbits were immunized with human, pig, and lamb serum. Each animal received 1 ml of each type in a separate site weekly for a total of 5 injections of each type. Ten days after the last injection, blood was drawn from each rabbit, and the serum tested in the Ouchterlony plate against human, pig, and lamb serum. Serum from each of the 5 control rabbits demonstrated dense precipitation lines against the sera of the other 3 species. Serum from 8 of the 9 experimental rabbits failed to demonstrate precipitation lines against human serum, but demonstrated dense lines against pig and lamb serum. Serum from one of the 9 experimental rabbits demonstrated lines against human serum also. These results demonstrate that it is feasible to produce in neonatal rabbits, substantial immune tolerance to the multiple antigens found in normal human blood. This may not be the case with other combinations of species.

This publication has 0 references indexed in Scilit: