Rapid and Simple Identification of Orientia tsutsugamushi from Other Group Rickettsiae by Duplex PCR Assay Using groEL Gene
Open Access
- 1 June 2005
- journal article
- research article
- Published by Wiley in Microbiology and Immunology
- Vol. 49 (6) , 545-549
- https://doi.org/10.1111/j.1348-0421.2005.tb03760.x
Abstract
In this study, two new duplex PCR methods based on the groEL gene were developed and investigated for the diagnosis of rickettsiae. The first duplex PCR assay amplified the 229‐bp and the 366‐bp DNAs of 6 strains including typhus group (TG) and spotted fever group (SFG) rickettsiae, and 5 scrub typhus group (STG) rickettsiae, respectively. The second duplex PCR assay amplified the 397‐bp and the 213‐bp DNAs of 6 Rickettsia strains and 5 STG strains. These duplex PCR methods could simultaneously perform the rapid identification of rickettsiae and the differential diagnosis of STG and other group rickettsiae in a single reaction.Keywords
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