Abstract
Summary: This study describes the influence of light, temperature, and moisture on N2‐fixing ability of Alnus glutinosa. Diurnal and day by day variations in the rate of acetylene reduction by excised root nodules were observed.Field‐grown A. glutinosa responded in C2H2‐reducing ability due to fluctuation in solar radiation or temperature or both when the plants were not under moisture stress. When solar radiation and temperature increased during the day, the rate of C2H2 reduction increased. Ample soil moisture was needed for nodules of A. glutinosa to be responsive to solar radiation or temperature.