EVIDENCE OF CYTOCIDAL EFFECTS OF PASTEURELLA-HAEMOLYTICA ON BOVINE PERIPHERAL-BLOOD MONONUCLEAR LEUKOCYTES

  • 1 January 1980
    • journal article
    • research article
    • Vol. 41  (10) , 1690-1693
Abstract
P. haemolytica had a pronounced cytocidal effect on bovine mononuclear leukocytes. Mononuclear leukocytes separated from venous blood on Ficoll-diatrizoate and incubated with P. haemolytica or Escherichia coli showed a significantly greater percentage cell death than did cells incubated with Staphylococcus epidermidis or controls. Neither heat-killed nor X-irradiated P. haemolytica caused a significant percentage cell death. The cytotoxic potential of P. haemolytica was dose related in that cell death among mononuclear leukocytes increased as the ratio of bacteria-to-cells increased. Enriched monocyte cultures showed a higher and less variable rate of cell death than either enriched lymphocyte or mixed leukocyte cultures.