Derivation of theoretical equations of the CO2 dissociation curve and the carbamate fraction in the Haldane effect.

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Abstract
The simultaneous Henderson-Hasselbach equations in plasma and red cell were solved to obtain the CO2 dissociation curve of oxygenated blood. To solve the above 2 equations the following equation was added, in which the relationship beween the intracellular (.DELTA.pHC) and the extracellular pH change (.DELTA.pHP) was defined as follows: pHC = (1 + .sigma.).DELTA.pHP, where 1 + .sigma. is a factor to be determined from experimental data on Donnan''s ratio for H+. From the solution, the ratio of bicarbonate shift to the CO2 quantity released out of or combined with Hb was calculated. The solution was validated by comparing the above ratio between the theoretical and experimental data. The CO2 contents calculated at 12 Torr in whole blood, red cell and plasma compartments show good agreement with the respective analyzed values. When the buffer values of Hb and plasma buffer protein were 70.0 and 7.5 mmol/(l plasma .cntdot. pH), respectively, .sigma. = -0.21 + 0.05 .cntdot. .DELTA.pHP, and the Donnan''s ratio for HCO3- was assumed to be 0.7 at pH = 7.33, the theoretical CO2 dissociation curve fitted well with the experimental curve. The CO2 dissociation curve of deoxygenated blood was expressed by adding the measured Haldane effect to the CO2 content of oxygenated blood. This additive characteristic in turn made it possible to estimate carbamate contribution in the Haldane effect.

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