A crowding factor causing spontaneous decapitation in cultures of the planarian Dugesia tigrina

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Abstract
Cultures of Dugesia tigrina maintained at densities of around 0.3 worms/ml or more show high frequencies of head injury. Histologically these injuries are seen to include the brain. The percentage of worms with head injuries is related to population density. The volume of culture water and time elapsed since feeding are also seen to influence the occurrence of injuries. The factor(s) concerned appear to be heat stable and dialyzable.

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