Headgroup conformation and lipid--cholesterol association in phosphatidylcholine vesicles: a 31P(1H) nuclear Overhauser effect study.
- 1 September 1975
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 72 (9) , 3477-3481
- https://doi.org/10.1073/pnas.72.9.3477
Abstract
The nuclear Overhauser effect has been observed in the nuclear magnetic resonance spectra of 31P. The information content of the nuclear Overhauser effect has been applied to the structure and dynamic properties of phosphatidylcholine vesicles. In the vesicles only 1/3 of the theoretical maximum nuclear Overhauser effect enhancement is observed. This result is accounted for by dipolar interactions between the N-methyl protons and the phosphate of phosphatidylcholine, and a correlation time for internal motion of 1.4 X 10(-9) sec. Addition of up to 30% cholesterol does not change the nuclear Overhauser effect enhancement or spin-lattice relaxation time of the vesicles. It is argued that the OH group of cholesterol is hydrogen bonded to the ester carbonyl oxygen of the phosphatidylcholine molecules.Keywords
This publication has 20 references indexed in Scilit:
- Frequency dependence of 31P NMR linewidths in sonicated phospholipid vesicles: Effects of chemical shift anisotropyFEBS Letters, 1974
- Cholesterol-phosphatidylcholine interactions in vesicle systems. Implication of vesicle size and proton magnetic resonance line-width changesBiochemistry, 1974
- An X-Ray and Vapour Pressure Study on Lecithin-Cholesterol-Water Interactions.Acta Chemica Scandinavica, 1974
- An interacting spin label study of the fluidizing and condensing effects of cholesterol on lecithin bilayersBiochimica et Biophysica Acta (BBA) - Biomembranes, 1973
- Phospholipid-cholesterol interaction. Kinetics of water permeability in liposomesBiochemistry, 1972
- Effect of sonication on the structure of lecithin bilayersBiochemistry, 1972
- Phosphorus and proton nuclear magnetic resonance studies in sarcoplasmic reticulum membranes and lipids. A comparison of phosphate and proton group mobilities in membranes and lipid bilayersBiochimica et Biophysica Acta (BBA) - Biomembranes, 1972
- Molecular dynamics of cerebroside—cholesterol and sphingomyelin—cholesterol interactions: Implications for myelin membrane structureFEBS Letters, 1972
- Magnetic resonance studies on membrane and model membrane systems: II. Phosphorus spectra and relaxation rates in dispersions of lecithinJournal of Supramolecular Structure, 1972
- A general synthesis of glycerophospholipidsBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1970