Production of the Soybean-Chlorosis Toxin by Rhizobium japonicum in Pure Culture
Open Access
- 1 September 1965
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 40 (5) , 931-933
- https://doi.org/10.1104/pp.40.5.931
Abstract
A chlorosis-inducing phytotoxin, formerly known to be produced only in soybean nodules, was found to be synthesized by several strains of R. japonicum in pure culture. Two of the strains have not been observed to induce chlorosis in soybeans, while 2 chlorosis-inducing strains failed to produce detectable quantities of the toxin in pure culture. Production of the phytotoxin by a selected strain was greatly affected by the culture medium. Maximum production per unit volume of culture was obtained with cells grown in yeast extract medium supplemented with casamino acids and harvested at the end of the logarithmic phase of growth. Procedures for isolating mg quantities of the phytotoxin are described.This publication has 1 reference indexed in Scilit: