Comparison of Two Pig Intestinal Brush Border Peptidases with the Corresponding Renal Enzymes

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Abstract
Intestinal dipeptidyl peptidase IV and .gamma.-glutamyltransferase were compared to the corresponding kidney enzymes with respect to immunological and electrophoretic properties. The influences of selected effectors on the 2 enzymes were also studied. The 2 kidney peptidases exhibited the reaction of total identity with the corresponding intestinal enzymes in immunodiffusion. The intestinal dipeptidyl peptidase IV and .gamma.-glutamyl-transferase showed the same inhibition patterns as the corresponding kidney enzymes and the acceptor specificity of the intestinal .gamma.-glutamyltransferase was identical to that of the kidney enzyme. The electrophoretic mobilities of dipeptidyl peptidase IV from the 2 organs differed greatly. The difference was almost abolished by treatment with neuraminidase, suggesting that the variation in mobility was due to different contents of sialic acid. The intestinal brush border peptidases, dipeptidyl peptidase IV and .gamma.-glutamyltransferase, may be closely related to the corresponding enzymes obtained from the kidney.