Production and specificity of a monoclonal anti-11-deoxycortisol antibody.

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Abstract
The production of a monoclonal antibody to 11-deoxycortisol is described. Spleen cells from BALB/c mice immunized with 4-(2-carboxyethylthio)-11-deoxycortisol linked to bovine serum albumin were fused with P3-NS1/1-Ag4-1 myeloma cells. A hybridoma clone (S.CET.M8.1.1) was established that secreted immunoglobulin G1 (IgG1) with a high affinity for 11-deoxycortisol (Ka=2 .times. 1010 M-1). The specificity of the monoclonal antibody (CET-M8) was assessed by cross-reaction studies with closely related steroids and by measuring the amount of 11-deoxycortisol in human plasma specimens by means of radioimmunoassay, in comparison with that of a conventional polyclonal antiserum. The results showed that the monoclonal antibody is reasonably specific and useful for the determination of plasma 11-deoxycortisol levels in the metyrapone test. Factors influencing the specificity of monoclonal antibodies to steroids are discussed.