Lysostaphin endopeptidase-catalysed transpeptidation reactions of the imino-transfer type
- 1 October 1977
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 167 (1) , 293-296
- https://doi.org/10.1042/bj1670293
Abstract
The glycylglycine endopeptidase in lysostaphin [of Staphylococcus staphylolyticus] can catalyze hydrolysis and transpeptidation reactions when acting on glycyl peptides. The ability of the enzyme to utilize dansyldiglycine (5-dimethylaminonaphthalene-1-sulfonylglycyl-glycine) as an acceptor molecule in transpeptidation reactions, although it is incapable of hydrolysing the peptide bond in this compound, indicates the enzyme must be capable of forming the equivalent of an imino-enzyme intermediate during the catalytic process.This publication has 12 references indexed in Scilit:
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