Effects of Neonatal Administration of Monosodium Glutamate on Somatotrophs and Growth Hormone Secretion in Prepubertal Male and Female Rats*

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Abstract
Female and male rats were injected with monosodium L-glutamate (MSG; 4 mg/g body wt) or 0.9% saline as neonates and then decapitated on days 35 and 40 of life, respectively. Trunk blood was collected for RIA [radioimmunoassay] of serum GH [growth hormone]. Anterior pituitary glands (APG) were bisected. One half was assayed for GH. The other half was placed in culture medium to study the basal GH release rate. Pituitary sections from additional rats were stained for GH, and morphometric analyses were performed on the GH cells. Treatment with MSG lowered serum GH levels and gland GH content in female but not in male rats. MSG did not alter the gland GH concentration or the basal GH release rate whether expressed per mg APG or per entire gland in either sex. The mean cross-sectional area of GH cells was reduced in either sex of MSG-treated rats. The numerical density of GH cells and the percentage of GH cells in APG were similar in saline- and MSG-treated rats of either sex. The volume density of GH cells was lower in MSG-treated male rats only. In prepubertal rats which had been given MSG as neonates, there is a sex difference in mean serum GH concentration and APG GH content; GH cell size is reduced in both sexes; and the individual GH cells contain normal amounts of GH in spite of their smaller size.

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