ASSAY OF LACTOGENIC HORMONE1

Abstract
The micro or local method of lactogenic hormone assay has been examined using 15 common pigeons for each assay. The hormone was injected over the symmetrically opposite side of the crop-sac in some of the assays in order to compare responses resulting from two hormone concentrations. In 4 assays, only one side was injected. The degree of proliferation of the crop-sac following injection of various levels of lactogenic hormone resulted in the establishment of a regression line, with a standard deviation of .0956, between log-dose per pigeon (X) and diameter (cm.) of proliferation obtained (Y) over a 31-fold dose range (.00072–.0224 mg. lactogenic hormone/bird). An index of precision (λ) of .11 was obtained. Responses resulting from injecting doses exceeding .0224 mg./bird or lower than .00072 mg./bird were difficult to measure.

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