Ketamine significantly reduces the migration of leukocytes through endothelial cell monolayers

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Abstract
To study the role of neutrophils in host defense, using human endothelial cells in migration studies in the presence of ketamine (0.3, 3, and 30 [micro sign]g/mL). Prospective, controlled study. University research laboratories. Seven independent experiments from different donors were done, investigating the influence of ketamine (0.3, 3, and 30 [micro sign]g/mL) to the migration of human leukocytes through human endothelial cell monolayers. Human endothelial cell monolayers and/or human leukocytes were preincubated with clinically relevant (3 [micro sign]g/mL), higher (30 [micro sign]g/mL), and lower (0.3 [micro sign]g/mL) concentrations of ketamine. The amount of leukocyte migration after 3 hrs was measured in a fluorometer. Human endothelial cells isolated from umbillcal veins were cultured on microporous membranes (polyethylene-terephthalat membranes) until they formed an endothelial cell monolayer. Leukocytes were separated by standard procedures. The migration of leukocytes through monolayers of endothelial cells under the clinically relevant concentration of ketamine was reduced to 59 +/-9.8% (SD) (p .05) when only monolayers of endothelial cells were treated with ketamine, and to 52 +/- 8.8% (p We investigated the cellular interaction between both cell systems, leukocytes and endothelial cells, simultaneously in the presence of ketamine. Ketamine is able to reduce significantly the migration of leukocytes through endothelial cell monolayers. The use of different dosages revealed a dose-dependent effect. The current model allowed treatment of one cell type, either leukocyte or endothelial cell. Ketamine inhibits the function of leukocytes more than the function of endothelial cells. (Crit Care Med 1998; 26:1545-1549)