Blood Preservation 33

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Abstract
Blood [from humans] storage in CPD[citrate-phosphate-dextrose]- adenine supplemented with 15 mM inosine and 10 mM phosphate gave 2,3-DPG [2,3-diphosphoglycerate] levels as high as 140% of normal for 6 wk of blood storage at 4.degree. C. Lower but normal 2,3-DPG levels maintained throughout 6 wk with inosine plus ribose. Ribose alone provided marginaly increased DPG maintenance over the control, but ribose with phosphate maintained 2,3-DPG levels above 70% of normal for 5 wk of storage and 2 wk longer than the control preservative. ATP levels were maintained at normal or above for 6 wk with phosphate plus ribose or inosine. 2,3-DPG maintenance is impaired by phosphate, unless inosine is also present. The ribose and inosine effects on 2,3-DPG maintenance are not additive. Phosphate also had an enhancement effect on ATP maintenance in the presence of either ribose or inosine.