MIGRATION INHIBITION OF HUMAN LEUKOCYTES MIXED WITH PHYTOHEMAGGLUTININ-PREINCUBATED MONONUCLEAR LEUKOCYTES
- 1 November 1975
- Vol. 30 (5) , 239-249
- https://doi.org/10.1111/j.1398-9995.1975.tb00200.x
Abstract
An in vitro test able to demonstrate phytohemagglutinin (PHA)-induced migration inhibition of human peripheral blood leukocytes was investigated. The migration inhibition was demonstrated by the agarose migration technique. Purified mononuclear leukocytes which had been incubated for 30 min with PHA, then washed and added to non-separated leukocytes induced migration inhibition of the mixed cell population. Since no PHA was added to the non-separated leukocytes, a direct PHA effect on polymorph migration was avoided. If the PHA-preincubated mononuclear leukocytes were heated at 56 degrees C for 30 min, their ability to cause migration inhibition was abolished. This suggests that the inhibition was caused by migration inhibitory factor (MIF) produced by the PHA-stimulated lymphocytes.Keywords
This publication has 8 references indexed in Scilit:
- SELECTIVE TRIGGERING OF HUMAN T AND B LYMPHOCYTES IN VITRO BY POLYCLONAL MITOGENSThe Journal of Experimental Medicine, 1974
- Effect of phytohaemagglutinin in the leucocyte migration inhibition test as a measure of cell-mediated immunityJournal of Clinical Pathology, 1974
- A BLASTOGENIC FACTOR IN UNIDIRECTIONAL MIXED CULTURES WITH X-IRRADIATED CELLSTransplantation, 1971
- Inhibition of Macrophage Migration by a Soluble Factor from Lymphocytes stimulated with PHA or ALSNature, 1971
- TUBERCULIN‐INDUCED MIGRATION INHIBITION OF HUMAN PERIPHERAL LEUCOCYTES IN AGAROSE MEDIUMAllergy, 1971
- LYMPHOCYTE ABNORMALITY IN CHRONIC MUCOCUTANEOUS CANDIDIASISThe Lancet, 1970
- Release of Skin Reactive Factor from Guinea-pig Lymphocytes by MitogensNature, 1970
- Lymphocyte Cytotoxicity in vitro: Activation and Release of a Cytotoxic FactorNature, 1968