Specificity of transport of neutral and basic amino acids in rat kidney

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Abstract
Competition for renal transport was studied in vivo in the rat. Proline, hydroxyproline, and glycine interacted as one group; lysine, arginine, and ornithine, and perhaps cystine, interacted as another. The specificity of mediated transport into kidney cortex slices was then studied. Estimates of Km and Ki values were determined using these amino acids, both as substrates and as competitive inhibitors. The data indicated that several systems are available for the uptake of the amino acids and glycine, rather than a single common system. Similarly, the basic amino acids were transported separately from cystine by multiple mediations rather than by a single common system. Therefore membrane transport system for amino acids appear to possess both individual and group specificities.