Inhibitors of poly(ADP‐ribose) polymerase enhance DNA strand breaks, excision repair, and sister chromatid exchanges induced by alkylating agents
- 1 January 1983
- journal article
- research article
- Published by Wiley in Environmental Mutagenesis
- Vol. 5 (4) , 515-525
- https://doi.org/10.1002/em.2860050402
Abstract
Benzamide and 3‐aminobenzamide, inhibitors of poly(ADP‐ribose) polymerase, synergistically enhanced the frequencies of unscheduled DNA synthesis and sister chromatid exchanges in Chinese hamster ovary (CHO) cells treated with N‐methyl‐N'‐nitro‐N‐nitrosoguanidine (MNNG). These inhibitors also increased methyl methanesulfonate (MMS)‐ or MNNG‐induced DNA strand breaks and significantly retarded the rejoining of strand breaks in CHO and HeLa S3 cells. These results suggest that poly(ADP‐ribose) is important in the repair of DNA damage after exposure to alkylating agents and exercises a regulatory role in stabilizing chromatin structure whenever strand breaks occur in DNA.Keywords
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