Variations of Natural 15N Abundances in Leguminous Plants and Nodule Fractions

Abstract
Natural 15N abundances of various tissues from twelve species of leguminous plants and those of nodule fractions from three species grown in Japan were determined by mass spectrometry. The 15N concentrations of non-nodular tissues of nodulated plants were close to the value of atmospheric N2. The nodules of soybean, mung bean, cowpea, kidney bean, adzuki bean, sword bean, white clover, Leucaena leucocephala, and horsegram showed highly elevated 15N concentrations, and those of pea had a slightly enriched 15N concentration compared to other tissue, while the nodules of peanut and lucerne had 15N values similar to those of their shoot tissues. The bacteroid fractions from horsegram, sword bean and peanut had higher 15N concentrations than the cytosol fractions.

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