Adenovirus-Cell Interactions Early After Infection: In vitro Characteristics and Tumourigenicity of Adenovirus Type 2-transformed Rat Liver Epithelial Cells

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Abstract
Cloned rat liver epithelial cells (clone C3) were semi-permissive for adenovirus type 2 (Ad-2) and non-permissive for adenovirus type 12 (Ad-12). Ad-2-infected C3 cells produced hexon and fiber proteins, but, at a multiplicity of infection of 20, a maximum virus yield of only 2.4 plaque-forming units/cell was obtained. By 48 h after infection with Ad-12, early virus proteins (major species 8000 and 60,000), but no late proteins (virus structural proteins), could be identified. Of 6 Ad-2-transformed epithelial lines isolated from clone C3 only 1 was tumorigenic in syngeneic rats; all 6 transformants produced tumors in athymic nude mice. There was a remarkable variation in the morphology of the Ad-2-transformed liver cells, ranging from an epithelial morphology similar to C3 cells to cells with a distinct lymphoid morphology. The in vitro and in vivo behavior of the Ad-2-transformed clone C3 cells, together with previous findings on the characteristics of Ad-12-transformed C3 cells, clearly show that the differences observed between Ad-2- and AD-12-transformed rat embryo cells were also observed in cloned rat liver epithelial cultures. The heterogeneity of Ad-2 transformation events apparently is not the result of the transformation in vitro of different types of target cell.

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