Effects of antibiotics on protein synthesis and degradation in primary cultures of rat hepatocytes
- 1 January 1981
- journal article
- Published by Springer Nature in In Vitro Cellular & Developmental Biology - Plant
- Vol. 17 (1) , 71-76
- https://doi.org/10.1007/bf02618033
Abstract
In primary hepatocyte cultures, maintained in a protein-free medium, streptomycin, penicillin, and Garamycin (gentamicin) all inhibited protein synthesis at concentrations above 0.1 mM. Some inhibition was also observed with the fungicide Mycostatin at 100 U/ml. Hepatocytic protein degradation was markedly inhibited by penicillin at concentrations above 0.1 mM, whereas streptomycin and Garamycin only showed slight inhibition at concentrations in excess of 1 mM. None of the antibiotics had any detectable effect on the structural integrity (viability) of the cells.Keywords
This publication has 25 references indexed in Scilit:
- Protein synthesis and protein degradation through the cell cycle of human NHIK 3025 cells in vitroExperimental Cell Research, 1979
- Alteration of ribosomal protein L6 in gentamicin-resistant strains of Escherichia coli. Effects on fidelity of protein synthesisBiochemistry, 1979
- Triphasic concentration effects of gentamicin on activity and misreading in protein synthesisBiochemistry, 1979
- The effect of protein degradation on cellular growth characteristicsJournal of Cellular Physiology, 1978
- Aminoglycoside antibiotics and eukaryotic protein synthesis: stimulation of errors in the translation of natural messengers in extracts of cultured human cellsBiochemistry, 1978
- Streptomycin‐ and viomycin‐induced conformational changes of ribosomes detected by iodinationFEBS Letters, 1978
- Fidelity of protein synthesis with chicken embryo mitochondrial and cytoplasmic ribosomesBiochemistry, 1974
- How penicillin kills bacteria: progress and problemsProceedings of the Royal Society of London. B. Biological Sciences, 1971
- Effect of streptomycin on bacteriophage φX174Biochemical and Biophysical Research Communications, 1965
- Nutrition Needs of Mammalian Cells in Tissue CultureScience, 1955