Comparison of media for isolation of salmonellae and shigellae from fecal specimens.
- 1 July 1971
- journal article
- research article
- Vol. 22 (1) , 17-22
Abstract
Five transport media, eight plating media, and three enrichment broth media for the isolation of salmonellae and shigellae were evaluated. Eight laboratories in widely separated regions of the United States participated in this evaluation by submitting 490 fecal specimens in the transport media provided. The results suggest that the newer transport media may not offer any advantage over the use of buffered glycerol-saline in the isolation of these enteric pathogens. Shigellae were best isolated by direct inoculation, whereas salmonellae were isolated in greater numbers after tetrathionate (without Brilliant Green) enrichment with subsequent culturing on the plating medium. The use of a variety of plating media is recommended for the recovery of a larger number of these enteric pathogens.This publication has 11 references indexed in Scilit:
- Comparison of media for direct isolation and transport of Shigellae from Fecal specimens.1970
- Isolation of Salmonellae and Shigellae from an artificial mixture of fecal bacteria.1969
- Isolation of Shigellae. VII. Comparison of gram-negative broth with Rappaport's enrichment broth.1969
- Comparison of xylose lysine deoxycholate agar and MacConkey agar for the isolation of salmonella and shigella from clinical specimens.1969
- A modified formula for the preparation of Stuart's Transport Medium.1967
- Isolation of shigellae. II. Comparison of plating media and enrichment broths.1965
- Disadvantages of the Rectal Swab in Diagnosis of DiarrhoeaBMJ, 1954
- The relative efficacy of culture media in the isolation of Shigella sonnei.1954
- The Public Health Laboratory Diagnosis of Enteric InfectionsThe American Journal of Tropical Medicine, 1950