The fractionation of calf thymus DNA by multiple formation of sedimentable complexes with homologous lysine-rlch histone KAP. Derivative melting curves and ultracentrifugatlon in CsCl concentration gradients
- 1 January 1974
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 1 (12) , 1675-1690
- https://doi.org/10.1093/nar/1.12.1675
Abstract
The process of fractionation of total calf thymus DNA using a step precipitation of DNA by means of increasing concentrations of the homologous histone KAP was investigated. In addition to the known fractions three so far undescribed ones/in thymus/,characterized by buoyant densities in CsCl equal 1.692, 1.706 and 1.728 g/ccm, were identified. Considerable amounts of preparations seriously enriched in individual satellite fractions were obtained. The ability of GC-rich satellite DNAs to form more soluble complexes with histone KAP is suggested as reason for the observed fractionation.Keywords
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