Abstract
The composition of the amino-acid pool of S. cerevisiae was markedly influenced by the amino-acid N source. The yeast tends to accumulate the amino acid supplied and those closely related to it metabolically. A relatively high concentration of glutamic acid is maintained in the pools of all cultures irrespective of the N source, reflecting the central role of glutamic acid in N metabolism. The total amino-acid pool concentration increases as a function of growth rate, although differences exist in the behavior of individual amino acids.

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