Multiple defects in antigen presentation and T cell development by mice expressing cytoplasmic tail–truncated CD1d
- 3 December 2001
- journal article
- research article
- Published by Springer Nature in Nature Immunology
- Vol. 3 (1) , 55-60
- https://doi.org/10.1038/ni740
Abstract
For members of the CD1 family of β2-microglobulin–associated lipid-presenting molecules, tyrosine-based motifs in the cytoplasmic tail and invariant chain (Ii) govern glycoprotein trafficking through endosomal compartments. Little is known about the intracellular pathways of CD1 trafficking and antigen presentation. However, in vitro studies with cells transfected with mutant CD1 that had a truncated cytoplasmic tail have suggested a role for these tyrosine motifs in some, but not all, antigenic systems. By introducing a deletion of the tyrosine motif into the germ line, and through homologous recombination in embryonic stem cells, we now describe knock-in mice with the CD1d cytoplasmic tail deleted. Despite adequate surface CD1d expression and the presence of Ii, these mutant mice showed multiple and selective abnormalities in intracellular trafficking, antigen presentation and T cell development, demonstrating the critical functions of the CD1d cytoplasmic tail motif in vivo.Keywords
This publication has 33 references indexed in Scilit:
- The Mouse Cd1d-Restricted Repertoire Is Dominated by a Few Autoreactive T Cell Receptor FamiliesThe Journal of Experimental Medicine, 2001
- THE CD1 SYSTEM: Antigen-Presenting Molecules for T Cell Recognition of Lipids and GlycolipidsAnnual Review of Immunology, 1999
- MOUSE CD1-SPECIFIC NK1 T CELLS: Development, Specificity, and FunctionAnnual Review of Immunology, 1997
- Positive selection of mouse NK1+ T cells by CD1-expressing cortical thymocytes.The Journal of Experimental Medicine, 1995
- CD1-Restricted T Cell Recognition of Microbial Lipoglycan AntigensScience, 1995
- CD1 Recognition by Mouse NK1 + T LymphocytesScience, 1995
- An invariant T cell receptor alpha chain is used by a unique subset of major histocompatibility complex class I-specific CD4+ and CD4-8- T cells in mice and humans.The Journal of Experimental Medicine, 1994
- Major histocompatibility complex class I related molecules control the development of CD4+8- and CD4-8- subsets of natural killer 1.1+ T cell receptor-alpha/beta+ cells in the liver of mice.The Journal of Experimental Medicine, 1994
- A Subset of CD4 + Thymocytes Selected by MHC Class I MoleculesScience, 1994
- CDlb restricts the response of human CD4−8−T lymphocytes to a microbial antigenNature, 1992