Rapid and efficient detection of genetic polymorphism in wheat through amplification by polymerase chain reaction
- 1 July 1990
- journal article
- research article
- Published by Springer Nature in Plant Molecular Biology
- Vol. 15 (1) , 169-171
- https://doi.org/10.1007/bf00017737
Abstract
The polymerase chain reaction (PCR) was used to amplify genomic DNA of several wheat genotypes. The oligonucleotides used as primers were the terminal sequences of a gamma-gliadin gene. The electrophoretic analysis of the PCR products showed specific bands which revealed both inter- and intra-specific genetic polymorphism among the examined genotypes. The technique is proposed as a very simple and efficient alternative to RFLP markers.This publication has 5 references indexed in Scilit:
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- The association between γ-gliadin 45 and gluten strength in durum wheat varieties: A direct causal effect or the result of genetic linkage?Journal of Cereal Science, 1984