PLATELET-ACTIVATING FACTOR AND ANALOGS - COMPARATIVE STUDIES WITH HUMAN-NEUTROPHILS AND RABBIT PLATELETS

  • 1 January 1983
    • journal article
    • research article
    • Vol. 39  (2) , 291-309
Abstract
Platelet-activating factor and 12 structural analogs stimulated rabbit platelets to aggregate and release [14C]-serotonin. They also caused human neutrophils to aggregate, degranulate and take up [3H]-deoxyglucose. Their respective potencies, which varied by 4-5 orders of magnitude, correlated highly (r .gtoreq. 0.93) in all assays. These compounds also selectively desensitized neutrophils to the degranulating actions of platelet-activating factor but not to C5a or a formylated oligopeptide. Three other analogs with structures quite similar to platelet-activating factor were unable to activate or desensitize the cells. The structure-activity relations of the analogs in several assays of platelet and neutrophil function were similar, and they stimulated neutrophils by a common activation mechanism that differed from those used by C5a or formylated oligopeptides. Platelet-activating factor apparently activates and desensitizes various target cells through stereospecific receptors. Apparently, these putative receptors on neutrophils and platelets have similar structural specificities for platelet-activating factor and its analogs.

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