Spontaneous Metastasis of Cells of the Human Prostate Carcinoma Cell Line PC-3 in Athymic Nude Mice

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Abstract
Spontaneous metastasis and extensive invasiveness were observed in athymic nude mice injected with human prostatic carcinoma cells of the PC-3 line or heterotransplants of nude mouse supported PC-3 tumor. In 3 experimental series, 60, 63 and 50% of the nude mice receiving s.c. inoculations of PC-3 cells or tumor heterotransplants developed 1 or more lymphatic tumor metastases. Examination of metaphase-arrested cells recovered from the metastatic sites confirmed the tumor origin as human in each case. Cells recovered from 1 nude mouse supported subline, MPC-30-10, frequently exhibited double minute chromosomes in addition to the typical PC-3 chromosomal profile. The observations provide the formation for a study of the relationship between prostate carcinoma cell characteristics and lymphatic metastasis in the nude mouse.