Vitronectin receptor has a role in bone resorption but does not mediate tight sealing zone attachment of osteoclasts to the bone surface.
Open Access
- 15 November 1991
- journal article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 115 (4) , 1179-1186
- https://doi.org/10.1083/jcb.115.4.1179
Abstract
During bone resorption, osteoclasts form a tight attachment, the sealing zone, around resorption lacunae. Vitronectin receptor has previously been shown to be expressed in osteoclasts and it has been suggested that it mediates the tight attachment at the sealing zone. In this study we have shown that glycine-arginine-glycine-aspartic acid-serine pentapeptide inhibits bone resorption by isolated osteoclasts and drastically changes the morphology of the osteoclasts. When the vitronectin receptor was localized by immunofluorescence in rat and chicken osteoclasts cultured on bone slices, it was found to be distributed throughout the osteoclast cell membrane except in the sealing zone areas. Immunoperoxidase staining of rat bone sections at the light microscopical level also revealed intense staining of the cell membrane with occasional small unstained areas, probably corresponding to the sealing zones. Immunoelectron microscopy confirmed the results obtained by light microscopy showing specific labeling only at the ruffled borders and basolateral membranes (0.82 and 2.43 gold particles/microns of membrane, respectively), but not at the sealing zone areas (0.06 gold particles/microns of membrane). Both alpha v and beta 3 subunits of the vitronectin receptor were similarly localized. These results strongly suggest that, although the vitronectin receptor is important in the function of osteoclasts, it is not mediating the final sealing zone attachment of the osteoclasts to the mineralized bone surface.Keywords
This publication has 32 references indexed in Scilit:
- Arginine-glycine-aspartic acid- and fibrinogen gamma-chain carboxyterminal peptides inhibit platelet adherence to arterial subendothelium at high wall shear rates. An effect dissociable from interference with adhesive protein binding.Journal of Clinical Investigation, 1990
- Echistatin is a potent inhibitor of bone resorption in culture.The Journal of cell biology, 1990
- The human integrin VLA-2 is a collagen receptor on some cells and a collagen/laminin receptor on others.Proceedings of the National Academy of Sciences, 1989
- Perspectives: Adhesion receptors in boneJournal of Bone and Mineral Research, 1989
- The osteoclast functional antigen, implicated in the regulation of bone resorption, is biochemically related to the vitronectin receptor.The Journal of cell biology, 1989
- The function of multiple extracellular matrix receptors in mediating cell adhesion to extracellular matrix: preparation of monoclonal antibodies to the fibronectin receptor that specifically inhibit cell adhesion to fibronectin and react with platelet glycoproteins Ic-IIa.The Journal of cell biology, 1988
- Influence of stereochemistry of the sequence Arg-Gly-Asp-Xaa on binding specificity in cell adhesion.Journal of Biological Chemistry, 1987
- New Perspectives in Cell Adhesion: RGD and IntegrinsScience, 1987
- Integrins: A family of cell surface receptorsCell, 1987
- Arg-Gly-Asp: A versatile cell recognition signalCell, 1986