Characterization by1H NMR of glycosidic conformations in the tetramolecular complex formed by d(GGTTTTTGG)
Open Access
- 11 September 1991
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 19 (17) , 4619-4622
- https://doi.org/10.1093/nar/19.17.4619
Abstract
We have conducted two dimensional NOESY studies on the molecule d(G2T5G2) to characterize the structure of the tetramolecular complex previously identified by calorimetric and spectroscopic studies (1). Analysis of the NOE and exchange cross peaks observed in the NOESY spectra establishes the formation of structured conformations at low temperature (5°C). Significantly, within each strand of these structured conformations, the G1 and G8 residues adopt syn glycosldic torsion angles, while the G2 and G9 residues adopt anti glycosidic torsion angles. Consequently, any structure proposed for the tetramolecular complex of d(G2T5G2) must have alternating G(syn) and G(anti) glycosidlc torsion angles within each strand. The implications of this observation for potential structures of the tetramolecular complex of d(G2T5G2) are discussed.Keywords
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