Exoerythrocytic Development of Plasmodium Gallinaceum Sporozoites In A Chicken Fibroblast Cell Line and Inhibition of the Cell Invasion By Specific Anti‐Sporozoite Monoclonal Antibodies

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Abstract
Cultivation of the Plasmodium gallinaceum exoerythrocytic forms from sporozoites was attempted in three diferent cell lines: HEPG2‐A16 (from a human hepatoma), VERO (monkey kidney epithelial cells) and SL‐29 (chicken embryo fibroblast cells). the sporozoites in vaded all three cell types but their development into exoerythrocytic forms ocurred only in the SL‐29 cells. In the presence of specific monoclonal antibodies against the major circumsporozoite protein, there were varying degrees of inhibition of parasite invasion of the SL‐29 cells. of seven monoclonal antibodies tested, two completely inhibited cell invasion at high concentrations and caused intense inhibition at concentrations as low as 2.5 μg/ml, four caused intense inhibition at these various concentrations, and one had no effect on sporozoite invasion.