Conversion of Unc104/KIF1A Kinesin into a Processive Motor After Dimerization
- 27 September 2002
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 297 (5590) , 2263-2267
- https://doi.org/10.1126/science.1073386
Abstract
Unc104/KIF1A belongs to a class of monomeric kinesin motors that have been thought to possess an unusual motility mechanism. Unlike the unidirectional motion driven by the coordinated actions of the two heads in conventional kinesins, single-headed KIF1A was reported to undergo biased diffusional motion along microtubules. Here, we show that Unc104/KIF1A can dimerize and move unidirectionally and processively with rapid velocities characteristic of transport in living cells. These results suggest that Unc104/KIF1A operates in vivo by a mechanism similar to conventional kinesin and that regulation of motor dimerization may be used to control transport by this class of kinesins.Keywords
This publication has 17 references indexed in Scilit:
- Direct Visualization of the Movement of the Monomeric Axonal Transport Motor UNC-104 along Neuronal Processes in LivingCaenorhabditis elegansJournal of Neuroscience, 2001
- Switch-based mechanism of kinesin motorsNature, 2001
- The Way Things Move: Looking Under the Hood of Molecular Motor ProteinsScience, 2000
- Mechanism of the single-headed processivity: Diffusional anchoring between the K-loop of kinesin and the C terminus of tubulinProceedings of the National Academy of Sciences, 2000
- 15 Å Resolution Model of the Monomeric Kinesin Motor, KIF1ACell, 2000
- Single-Molecule Behavior of Monomeric and Heteromeric KinesinsBiochemistry, 1999
- A Processive Single-Headed Motor: Kinesin Superfamily Protein KIF1AScience, 1999
- THE DESIGN PLAN OF KINESIN MOTORSAnnual Review of Cell and Developmental Biology, 1997
- The neuron-specific kinesin superfamily protein KIF1A is a uniqye monomeric motor for anterograde axonal transport of synaptic vesicle precursorsCell, 1995
- The C. elegans unc-104 4 gene encodes a putative kinesin heavy chain-like proteinNeuron, 1991