CHANGES IN SERUM TOTAL CREATINE-PHOSPHOKINASE (CPK) AND ITS ISOENZYMES CAUSED BY EXPERIMENTAL LIGATION OF THE SUPERIOR MESENTERIC-ARTERY

  • 1 January 1981
    • journal article
    • research article
    • Vol. 193  (4) , 499-505
Abstract
The changes in serum total CPK [creatine phosphokinase] and its isoenzymes were not delineated in acute mesenteric infarction. Measurement of serum CPK levels could conceivably be a useful diagnostic test for bowel infarction. Changes in serum CPK levels were assessed in bowel infarction in dogs, using sham operation and talc peritonitis as controls. Laparotomies were performed in 20 dogs, and each was assigned randomly to 1 of 3 groups: those having laparotomy (LAP), talc peritonitis (PER) and superior mesenteric artery infarction (MAI). Mixed venous blood samples were obtained from all subjects for 30 h after surgery. All animals were killed. Complete autopsies were performed. confirmation of infarction and determination of its extent were obtained through gross and microscopic examination of the gut in canines subjected to arterial infarction. Total serum CPK levels were determined by spectrophotometric analysis. Agarose gel electrophoresis was used to determine the levels of each of the isoenzymes. Significant elevations of CPK and CPK-MM occurred 9 h after injury. CPK-BB reached maximum elevation by 6 h, while CPK-MB did not reach its maximum until 24 h after injury. Total CPK and its isoenzymes apparently become elevated in the serum of canines subjected to experimental superior mesenteric artery infarction. That CPK-BB elevations peak in the first 12 h after injury may be of particular diagnostic significance.