QUANTITATIVE ASSAY OF SPECIFIC PROTEOLYTIC ACTIVITY IN FOOD PROTEIN HYDROLYZATES BY SINGLE RADIAL IMMUNODIFFUSION
- 1 January 1978
- journal article
- research article
- Published by Wiley in Journal of Food Science
- Vol. 43 (1) , 141-142
- https://doi.org/10.1111/j.1365-2621.1978.tb09754.x
Abstract
The increasing use of proteolytic enzymes in food production has established the need for a quantitative and sensitive assay for proteolytic activity in protein foods. The classical methods are not suitable because of the presence of large amounts of proteins and peptides. A quantitative procedure based on single radial immunodiffusion was developed and found to be applicable to the determination of subtilisin Carlsberg activity in protein hydrolyzates. Less than 0.5 ppm subtilisin Carlsberg could be detected in a 7% soy protein hydrolyzate. With minor modifications the method should be applicable to many of the other enzymes used in food processing.This publication has 9 references indexed in Scilit:
- Immunochemical, chromatographic, and milk-clotting activity measurements for quantification of milk-clotting enzymes in bovine rennetsJournal of Dairy Research, 1976
- Agarose electrophoresis of subtilisin Carlsberg in veronal buffer at pH 6.5Analytical Biochemistry, 1976
- Subtilopeptidase A isoenzyme system. Interaction with serum components and its importance for quantitative immunoelectrophoresisBiochemical Journal, 1975
- 20. Carbamylated Antibodies Used for Quantitation of Human IgG. A Routine MethodScandinavian Journal of Immunology, 1973
- Electroimmuno AssayScandinavian Journal of Clinical and Laboratory Investigation, 1972
- Immunochemical quantitation of antigens by single radial immunodiffusionImmunochemistry, 1965
- Immunochemical quantitation of antigens by single radial immunodiffusionMolecular Immunology, 1965
- THE ESTIMATION OF PEPSIN, TRYPSIN, PAPAIN, AND CATHEPSIN WITH HEMOGLOBINThe Journal of general physiology, 1938