Carbon Source and Micronutrient Requirements of the Aquatic Phycomycete, Catenaria anguillulae Sorokin

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Abstract
The requirements of the aquatic Phycomycete, Catenaria anguillulae were analysed in liquid, shake cultures using a standardized zoospore inoculum. Growth was determined by measuring mycelial dry weight and rate of production of titratable acid. D-glucose was the best carbon source and had an optimum concentration of 166 mM of carbon for the medium used. When other carbon sources were supplied, only those related to glucose (fructose and mannose) or composed of glucose units with an alpha-linkage (maltose, glycogen, and starch) were readily utilized. Lactic acid was determined qualitatively as an end-product of carbon metabolism. The optimum level of phosphate was 1.0 mM. The optimum concentration of EDTA was 0.032 mM. Of the chelated cations included in the medium only the omission of iron, zinc, calcium, or magnesium reduced growth. Concentrations of calcium below 0.4 mM and of magnesium below 0.2 mM were limiting; whereas, concentrations of both ions up to 1 mM were non-toxic.

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