G protein βγ directly regulates SNARE protein fusion machinery for secretory granule exocytosis
Top Cited Papers
- 20 March 2005
- journal article
- research article
- Published by Springer Nature in Nature Neuroscience
- Vol. 8 (4) , 421-425
- https://doi.org/10.1038/nn1423
Abstract
The activation of G protein–coupled receptors (GPCRs) can result in an inhibition of Ca2+-dependent hormone and neurotransmitter secretion. This has been attributed in part to G protein inhibition of Ca2+ influx. However, a frequently dominant inhibitory effect, of unknown mechanism, also occurs distal to Ca2+ entry. Here we characterize direct inhibitory actions of G protein βγ (Gβγ) on Ca2+-triggered vesicle exocytosis in permeable PC12 cells. Gβγ inhibition was rapid (2+-dependent synaptotagmin binding. Here we show inhibitory coupling between GPCRs and vesicle exocytosis mediated directly by Gβγ interactions with the Ca2+-dependent fusion machinery.Keywords
This publication has 25 references indexed in Scilit:
- G Protein βγ Subunit-Mediated Presynaptic Inhibition: Regulation of Exocytotic Fusion Downstream of Ca 2+ EntryScience, 2001
- Molecular mechanisms and regulation of insulin exocytosis as a paradigm of endocrine secretionEuropean Journal of Biochemistry, 1999
- PRESYNAPTIC RECEPTORSAnnual Review of Pharmacology and Toxicology, 1998
- Mechanisms of inhibition of insulin releaseAmerican Journal of Physiology-Cell Physiology, 1996
- A novel 145 kd brain cytosolic protein reconstitutes Ca2+-regulated secretion in permeable neuroendocrine cellsCell, 1992
- Regulatory Role of the GTP‐Binding Protein, Go, in the Mechanism of Exocytosis in Adrenal Chromaffin CellsJournal of Neurochemistry, 1992
- Evidence That Receptor‐Linked G Protein Inhibits Exocytosis by a Post‐Second‐Messenger Mechanism in AtT‐20 CellsJournal of Neurochemistry, 1990
- A neuromodulator of synaptic transmission acts on the secretory apparatus as well as on ion channelsNature, 1989
- Calcium-stimulated catecholamine release from .alpha.-toxin-permeabilized PC12 cells: biochemical evidence for exocytosis and its modulation by protein kinase C and G proteinsBiochemistry, 1987
- Guanine nucleotides and Ca‐dependent exocytosisFEBS Letters, 1985