THE EFFECT OF LIGNOCAINE ON PHA-STIMULATED HUMAN LYMPHOCYTE TRANSFORMATION

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Abstract
The effect of lignocaine on in-vitro phytohemagglutinin (PHA)-induced lymphocyte transformation was determined by the incorporation of tritiated thymidine into nucleic acid. Concentrations of lignocaine within the therapeutic range (1–10 μg/ml) did not significantly inhibit lymphocyte transformation. Significant inhibition was observed with 50 and 100 μg/ml concentrations of lignocaine (P<0.05). One thousand μg/ml lignocaine was lethal to lymphocytes. The inhibitory effect of normal blood concentrations of lignocaine on lymphocyte transformation is less than that reported for usual anaesthetic concentrations of halothane and some barbiturates.