Functional immunoglobulin M production after transfection of cloned immunoglobulin heavy and light chain genes into lymphoid cells.
- 1 October 1983
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 80 (20) , 6351-6355
- https://doi.org/10.1073/pnas.80.20.6351
Abstract
The rearranged immunoglobulin heavy (mu) and light (kappa) chain genes cloned from the Sp6 hybridoma cell line producing immunoglobulin M specific for the hapten 2,4, 6-trinitrophenyl were inserted into the transfer vector pSV2-neo and introduced into various plasmacytoma and hybridoma cell lines. The transfer of the mu and kappa genes resulted in the production of pentameric, hapten-specific, functional IgM.This publication has 40 references indexed in Scilit:
- Detection of specific sequences among DNA fragments separated by gel electrophoresisPublished by Elsevier ,2006
- Selective extraction of polyoma DNA from infected mouse cell culturesPublished by Elsevier ,2004
- Structural Basis of Antibody FunctionAnnual Review of Immunology, 1983
- Transfer of a cloned immunoglobulin light-chain gene to mutant hybridoma cells restores specific antibody productionNature, 1983
- Expression of mouse immunoglobulin genes in monkey cellsNature, 1982
- The cloning and reintroduction into animal cells of a functional CAD gene, a dominant amplifiable genetic markerCell, 1981
- Molecular cloning of an immunoglobulin kappa constant gene from NZB mouseGene, 1981
- The synthesis and processing of the messenger RNAs specifying heavy and light chain immunoglobulins in MPC-11 cellsCell, 1978
- Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase IJournal of Molecular Biology, 1977
- Derivation of specific antibody‐producing tissue culture and tumor lines by cell fusionEuropean Journal of Immunology, 1976