Abstract
In vitro initiation and development of shoot-buds of walnuts (Juglans regia L.) was obtained on a defined medium using seedlings as a primary explants. Benzylamino purine (BA) at 40 μM induced multiple shoot formation derived from an abnormal conical shoot. Periodic subculture on a fresh media with 0.4 μM of both indolebutyric acid (IBA) and BA resulted in shoots multiplication.

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