6β-Hydroxycortisol in random urine samples as an indicator of enzyme induction

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Abstract
6.beta.-Hydroxycortisol (6.beta.-OHF) is a major cortisol metabolite formed primarily in the hepatic reticulum by mixed-function oxygenases. Changes in 6.beta.-OHF may be used to detect enzyme induction by drugs or disease. Excretion of 6.beta.-OHF must be corrected for change in urinary free cortisol (FF) excretion to allow for changes in cortisol production. The absence of circadian changes in 6.beta.-OHF/FF ratios is reported. Random measurements of 6.beta.-OHF/FF ratios may be used to detect enzyme induction. This approach greatly simplifies noninvasive screening for enzyme induction in humans.