Monoclonal antibody to a protein of the nucleus and mitotic spindle of mammalian cells
- 1 August 1985
- journal article
- research article
- Published by Springer Nature in Chromosoma
- Vol. 92 (4) , 297-303
- https://doi.org/10.1007/bf00329813
Abstract
We report here the isolation of a monoclonal antibody, J17, that reacts with a conserved vertebrate protein antigen that is present in the spindle apparatus during mitosis but found within the nucleus during interphase. Immunofluorescence microscopy demonstrates that the J17 antigen is found in numerous punctate regions that are distinct from nucleoli. Furthermore, this antigen is not directly associated with kinetochores, the nuclear envelope, or with metaphase chromosomes. — Antibody J17 immunoprecipitates a single polypeptide of very high molecular weight (over 250000) from K562 human erythroleukemia cells pulse-labeled with 14C-leucine. This polypeptide is converted quantitatively to a stable 220-kilodalton product within one cellular generation. We discuss the possible relevance of this processing event for transport into the nucleus. The J17 antigen is synthesized throughout the cell cycle in Chinese hamster ovary cells.This publication has 21 references indexed in Scilit:
- Targeting of E. coli β-galactosidase to the nucleus in yeastCell, 1984
- Human anticentromere antibodies: Distribution, characterization of antigens, and effect on microtubule organizationCell, 1983
- Karyophilic proteins: polypeptides synthesized in vitro accumulate in the nucleus on microinjection into the cytoplasm of amphibian oocytes.Proceedings of the National Academy of Sciences, 1982
- A polypeptide domain that specifies migration of nucleoplasmin into the nucleusCell, 1982
- A dot-immunobinding assay for monoclonal and other antibodiesAnalytical Biochemistry, 1982
- A microtubule-associated protein in the mitotic spindle and the interphase nucleusNature, 1982
- Erythrocyte ankyrin: immunoreactive analogues are associated with mitotic structures in cultured cells and with microtubules in brain.Proceedings of the National Academy of Sciences, 1981
- The nuclear envelope lamina is reversibly depolymerized during mitosisCell, 1980
- Separation of human lymphocytes and monocytes by velocity sedimentation at unit gravityJournal of Immunological Methods, 1979
- Chromatin. Its isolation from cultured mammalian cells with particular reference to contamination by nuclear ribonucleoprotein particlesBiochemistry, 1973