Noninvasive Imaging of Quantum Dots in Mice
Top Cited Papers
- 30 December 2003
- journal article
- research article
- Published by American Chemical Society (ACS) in Bioconjugate Chemistry
- Vol. 15 (1) , 79-86
- https://doi.org/10.1021/bc034153y
Abstract
Quantum dots having four different surface coatings were tested for use in in vivo imaging. Localization was successfully monitored by fluorescence imaging of living animals, by necropsy, by frozen tissue sections for optical microscopy, and by electron microscopy, on scales ranging from centimeters to nanometers, using only quantum dots for detection. Circulating half-lives were found to be less than 12 min for amphiphilic poly(acrylic acid), short-chain (750 Da) methoxy-PEG or long-chain (3400 Da) carboxy-PEG quantum dots, but approximately 70 min for long-chain (5000 Da) methoxy-PEG quantum dots. Surface coatings also determined the in vivo localization of the quantum dots. Long-term experiments demonstrated that these quantum dots remain fluorescent after at least four months in vivo.Keywords
This publication has 10 references indexed in Scilit:
- Multiplexed SNP genotyping using the QbeadTM system: a quantum dot-encoded microsphere-based assayNucleic Acids Research, 2003
- Effect of pegylation on pharmaceuticalsNature Reviews Drug Discovery, 2003
- Long-term multiple color imaging of live cells using quantum dot bioconjugatesNature Biotechnology, 2002
- Nanoparticles in cancer therapy and diagnosisAdvanced Drug Delivery Reviews, 2002
- PEGylated antibodies and antibody fragments for improved therapy: a reviewAdvanced Drug Delivery Reviews, 2002
- Targeting Cell Surface Receptors with Ligand-Conjugated NanocrystalsJournal of the American Chemical Society, 2002
- Pegylation: engineering improved pharmaceuticals for enhanced therapyCancer Treatment Reviews, 2002
- PEG drugs: an overviewJournal of Controlled Release, 2001
- Near-infrared fluorescent dyes for enhanced contrast in optical mammography: phantom experimentsJournal of Biomedical Optics, 2001
- ‘Stealth’ corona-core nanoparticles surface modified by polyethylene glycol (PEG): influences of the corona (PEG chain length and surface density) and of the core composition on phagocytic uptake and plasma protein adsorptionColloids and Surfaces B: Biointerfaces, 2000