The effect of the 3′-terminal monophosphate group on the metal-ion-promoted hydrolysis of the phosphodiester bonds of short oligonucleotides

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Abstract
The effect of 3′-terminal monophosphate group on the metal-ion-promoted hydrolysis of the phosphodiester bonds of oligonucleotides has been studied. For this purpose, the rate constants for the hydrolysis of the following oligomers in the presence of Zn2+ and its 1,5,9-triazacyclododecane chelate, Zn2+[12]aneN3, have been determined: (i) ApUpUp(2′) and ApUpUp(3′), (ii) Up(Tp)nT (n= 0–4 and 7), (iii) Up(Tp)nTp (n= 0–4 and 7). The results obtained are used to propose a mechanism for the Zn2+ and Zn2+[12]aneN3 promoted hydrolysis of polynucleotides.